5 minutes with Dr. Klaus Weisshart & Dr. Kirstin Elgass

Kirstin:  ZEISS Lattice Lightsheet 7 is a turn-key system which can be operated by non-experts and can be calibrated in just minutes. Changing the sheet is also just a matter of a click - the system allows for simultaneous illumination with three sheets of different wavelengths.

Klaus:  In contrast to other Lattice Lightsheet implementations, our ZEISS Lattice Lightsheet 7 is compatible with standard sample carriers (dishes, multi-well, slides), so there’s no need to adjust sample preparation from other high-resolution microscopy techniques.

Kirstin:  Observation of samples is also contamination free - and with the 5-axis stage, that can also accommodate long travel ranges and oblique illumination, sample finding is greatly accelerated.

Kirstin:  Lattice Lightsheet is the only technology that allows you to image fluorescently labelled live cells/organisms for up to multiple days.

Klaus:  It’s also the most sample-friendly method with low photobleaching and phototoxicity, making it ideal for otherwise light sensitive cells and organisms. The system allows for fast volume imaging with an outstanding signal-to-noise ratio (SNR) at subcellular resolution.

Klaus:  Lightsheet 7 with its horizontal setup is made for live model organisms (from early embryonic stages to the adult organism), plant studies and cleared organs and organisms. It is ideal for larger specimen exceeding 100 μm in its size.

Lattice Lightsheet 7 with its inverted set up is ideal for cell culture, early embryonic stages of model organisms, thin tissues and all specimen smaller than 100 μm in size. The Lattice Lightsheet 7 also provides you the longest and thinnest light sheets, compared to Gausian beams used in Lightsheet 7 to achieve better sectioning.

There is very little overlap in regard to sample size and sample preparation.

Lattice light sheet microscopy overcomes the limitations of Gaussian beams (limited optical sectioning, limited field of view) and Bessel beams (strong rings, excitation of out-of-focus fluorescence) by generating long and thin light sheets to achieve subcellular resolution.